Journal: Analytical Chemistry

Article Title: Electrochemical Genosensing of Overexpressed GAPDH Transcripts in Breast Cancer Exosomes

doi: 10.1021/acs.analchem.2c04773

Figure Lengend Snippet: (A) Characterization by NTA of purified exosomes derived from the MCF7 breast cancer cell line. (B) Cryo-TEM images (i) and (ii) of purified exosomes at an acceleration voltage of 200 kV. (C) Confocal microscopy images and (D) flow cytometry study for the (i) MCF7 breast cancer cell line and (ii) their exosomes covalently immobilized on MPs. For confocal microscopy, DNA appears blue, magnetic particles in green color, while the exosome protein membrane in red color. For flow cytometry, in all cases, the negative controls (obtained with incubation with the secondary antimouse-Cy5) are shown in blue, while the positive CD326 and CD81 samples obtained with the incubation with the antiCD326 and antiCD81 primary antibodies, are shown in yellow and red, respectively. The negative control obtained with the exosomes-modified MPs without any labeling is shown in gray in panel D (ii).

Article Snippet: Tosyl-activated magnetic particles (MPs) (Dynabeads M450 Tosylactivated, ref. 14013), MPs modified with EpCAM antibody (antiCD326-MPs, Dynabeads Epithelial Enrich, ref. 16102), MPs modified with poly(dT) (polydT-MPs, Dynabeads Oligo(dT)25, ref. 61002), MPs modified with streptavidin (strep-MPs, Dynabeads MyOne Streptavidin T1, ref. 65601), mouse monoclonal antibody antiCD81 (ref. 10630D), and BCA protein assay kit (ref. 23225) were purchased from Thermo Fisher Scientific (MA, US).

Techniques: Purification, Derivative Assay, Confocal Microscopy, Flow Cytometry, Incubation, Negative Control, Modification, Labeling

Journal: Analytical Chemistry

Article Title: Electrochemical Genosensing of Overexpressed GAPDH Transcripts in Breast Cancer Exosomes

doi: 10.1021/acs.analchem.2c04773

Figure Lengend Snippet: Electrochemical genosensing of GAPDH transcripts from (A) MCF7 cells ranging from 50 to 5000 cells mL –1 and (B) their exosomes ranging from 100 to 4.0 × 10 4 exosomes μL –1 , according to NTA counting. In all cases, the cells and exosomes were lysed preconcentrated by IMS using antiCD81-MPs (▲) and antiCD326-MPs (●), followed by double-tagging RT-PCR on poly(dT)-MPs. The error bars show the standard deviation for n = 3.

Article Snippet: Tosyl-activated magnetic particles (MPs) (Dynabeads M450 Tosylactivated, ref. 14013), MPs modified with EpCAM antibody (antiCD326-MPs, Dynabeads Epithelial Enrich, ref. 16102), MPs modified with poly(dT) (polydT-MPs, Dynabeads Oligo(dT)25, ref. 61002), MPs modified with streptavidin (strep-MPs, Dynabeads MyOne Streptavidin T1, ref. 65601), mouse monoclonal antibody antiCD81 (ref. 10630D), and BCA protein assay kit (ref. 23225) were purchased from Thermo Fisher Scientific (MA, US).

Techniques: Reverse Transcription Polymerase Chain Reaction, Standard Deviation

Journal: Journal of Experimental Botany

Article Title: Transcriptional analysis of the Arabidopsis ovule by massively parallel signature sequencing

doi: 10.1093/jxb/ers075

Figure Lengend Snippet: MPSS signatures in wild-type and sporocyteless ovules of Arabidopsis

Article Snippet: MPSS signature sequencing MPSS was performed as described in Brenner et al. (2000) by Lynx Therapeutics/Solexa (Hayward, CA, USA).

Techniques:

Journal: Journal of Experimental Botany

Article Title: Transcriptional analysis of the Arabidopsis ovule by massively parallel signature sequencing

doi: 10.1093/jxb/ers075

Figure Lengend Snippet: Quantitative distribution of MPSS-detected genes in the ovule of Arabidopsis

Article Snippet: MPSS signature sequencing MPSS was performed as described in Brenner et al. (2000) by Lynx Therapeutics/Solexa (Hayward, CA, USA).

Techniques:

Journal: Journal of Experimental Botany

Article Title: Transcriptional analysis of the Arabidopsis ovule by massively parallel signature sequencing

doi: 10.1093/jxb/ers075

Figure Lengend Snippet: Differences in the universe of genes which are candidates to be specifically expressed in the female gametophyte of Arabidopsis. Venn diagram illustrating the distribution of genes which are candidates to be expressed in the female gametophyte on the basis of four independent experiments conducted with three distinct mutant backgrounds (spl, dif1, and coa) and three different platforms (Affymetrix ATH1 microarray, whole genome TILING microarray, and MPSS). The corresponding type of platform and mutant background are indicated for each experiment. (This figure is available in colour at JXB online.)

Article Snippet: MPSS signature sequencing MPSS was performed as described in Brenner et al. (2000) by Lynx Therapeutics/Solexa (Hayward, CA, USA).

Techniques: Mutagenesis, Microarray